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Image Search Results
Journal: Frontiers in Molecular Biosciences
Article Title: Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Acinetobacter calcoaceticus–baumannii Complex and Associated Carbapenem Resistance
doi: 10.3389/fmolb.2021.659256
Figure Lengend Snippet: (A) shows the alignment of sequence of diverse isolates of A. baumannii ( N =4), A. pittii ( N = 4), and A. nosocomialis (N=2) and demonstrates that the sequences are conserved across diverse isolates of three species except few locations. (B) shows the alignment of our consensus ITS 16S–23S rRNA sequence with the sequence of other Acinetobacter species ( A. calcoaceticus , A. lwoffii , A. radioresistens , A. haemolyticus , and A. junii ). The presence of the non-conserved region at locations from 140 to 210 (where our two to three primers are aligned) demonstrates that our LAMP primers were not specific for other Acinetobacter species.
Article Snippet: The specificity of LAMP assay was also determined using 21 non-ACB complex species [ A. lwoffii (6), A. variabilis (3), A. ursingii (2), A. radioresistens (2), A. junii (2), A. schindleri (2), A. indicus (2), A. haemolyticus (1), and A. bereziniae (1)] and 10 non-
Techniques: Sequencing
Journal: Frontiers in Molecular Biosciences
Article Title: Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Acinetobacter calcoaceticus–baumannii Complex and Associated Carbapenem Resistance
doi: 10.3389/fmolb.2021.659256
Figure Lengend Snippet: A universal consensus sequence of the ITS 16S–23S rRNA gene that was used to design the LAMP primers for detection of significant members of the Acinetobacter calcoaceticus–baumannii (ACB) complex. Red color bases signify the mixed bases used in place of different bases present in A. baumannii , A. nosocomialis , and A. pittii . The sequences sites for ITS Ab1 and Ab2 LAMP primers are designated by upper and lower horizontal arrows, respectively. Right and left arrows indicate sense and reverse complementary sequences that were used.
Article Snippet: The specificity of LAMP assay was also determined using 21 non-ACB complex species [ A. lwoffii (6), A. variabilis (3), A. ursingii (2), A. radioresistens (2), A. junii (2), A. schindleri (2), A. indicus (2), A. haemolyticus (1), and A. bereziniae (1)] and 10 non-
Techniques: Sequencing
Journal: Frontiers in Molecular Biosciences
Article Title: Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Acinetobacter calcoaceticus–baumannii Complex and Associated Carbapenem Resistance
doi: 10.3389/fmolb.2021.659256
Figure Lengend Snippet: Details of bacterial strains used in the study.
Article Snippet: The specificity of LAMP assay was also determined using 21 non-ACB complex species [ A. lwoffii (6), A. variabilis (3), A. ursingii (2), A. radioresistens (2), A. junii (2), A. schindleri (2), A. indicus (2), A. haemolyticus (1), and A. bereziniae (1)] and 10 non-
Techniques: